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Fig. 5. The subcutaneous tumor formation in nude mice and the expression levels of signature proteins in the NF-κΒ signaling pathway. A KEGG signaling pathway and GO analysis (BP, CC, MF) were performed based on ADAMDEC1-binding and related genes. B Western blot assay assessed the expression levels of <t>TNFR2,</t> p65, p-p65, IKB-α, and p-IKB-α proteins in the NF-κB signaling pathway in cell lines transfected with NF-κB activator 1 and /or siADAMDEC1. C Representative images of BALB/c nude mice and tumor masses were taken on day 38 after inoculation with ADAMDEC1 cells, with or without shRNA-mediated silencing of ADAMDEC1. D The weight and volume curves of subcutaneous tumors in nude mice (*P < 0.05). E HE staining showed changes in cancer cells and intracellular structures. Ki67 expression in the siADAMDEC1 group was detected by IHC staining (**P < 0.01).
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Fig. 5. The subcutaneous tumor formation in nude mice and the expression levels of signature proteins in the NF-κΒ signaling pathway. A KEGG signaling pathway and GO analysis (BP, CC, MF) were performed based on ADAMDEC1-binding and related genes. B Western blot assay assessed the expression levels of <t>TNFR2,</t> p65, p-p65, IKB-α, and p-IKB-α proteins in the NF-κB signaling pathway in cell lines transfected with NF-κB activator 1 and /or siADAMDEC1. C Representative images of BALB/c nude mice and tumor masses were taken on day 38 after inoculation with ADAMDEC1 cells, with or without shRNA-mediated silencing of ADAMDEC1. D The weight and volume curves of subcutaneous tumors in nude mice (*P < 0.05). E HE staining showed changes in cancer cells and intracellular structures. Ki67 expression in the siADAMDEC1 group was detected by IHC staining (**P < 0.01).
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Fig. 5. The subcutaneous tumor formation in nude mice and the expression levels of signature proteins in the NF-κΒ signaling pathway. A KEGG signaling pathway and GO analysis (BP, CC, MF) were performed based on ADAMDEC1-binding and related genes. B Western blot assay assessed the expression levels of <t>TNFR2,</t> p65, p-p65, IKB-α, and p-IKB-α proteins in the NF-κB signaling pathway in cell lines transfected with NF-κB activator 1 and /or siADAMDEC1. C Representative images of BALB/c nude mice and tumor masses were taken on day 38 after inoculation with ADAMDEC1 cells, with or without shRNA-mediated silencing of ADAMDEC1. D The weight and volume curves of subcutaneous tumors in nude mice (*P < 0.05). E HE staining showed changes in cancer cells and intracellular structures. Ki67 expression in the siADAMDEC1 group was detected by IHC staining (**P < 0.01).
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Clinical data in patients having diabetes mellitus (GI newly diagnosed DM, GII DM with metformin therapy) and control group
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Image Search Results


Fig. 5. The subcutaneous tumor formation in nude mice and the expression levels of signature proteins in the NF-κΒ signaling pathway. A KEGG signaling pathway and GO analysis (BP, CC, MF) were performed based on ADAMDEC1-binding and related genes. B Western blot assay assessed the expression levels of TNFR2, p65, p-p65, IKB-α, and p-IKB-α proteins in the NF-κB signaling pathway in cell lines transfected with NF-κB activator 1 and /or siADAMDEC1. C Representative images of BALB/c nude mice and tumor masses were taken on day 38 after inoculation with ADAMDEC1 cells, with or without shRNA-mediated silencing of ADAMDEC1. D The weight and volume curves of subcutaneous tumors in nude mice (*P < 0.05). E HE staining showed changes in cancer cells and intracellular structures. Ki67 expression in the siADAMDEC1 group was detected by IHC staining (**P < 0.01).

Journal: Scientific reports

Article Title: ADAMDEC1 promotes the malignant progression of cholangiocarcinoma by regulating NF-κB signaling pathway.

doi: 10.1038/s41598-025-85241-6

Figure Lengend Snippet: Fig. 5. The subcutaneous tumor formation in nude mice and the expression levels of signature proteins in the NF-κΒ signaling pathway. A KEGG signaling pathway and GO analysis (BP, CC, MF) were performed based on ADAMDEC1-binding and related genes. B Western blot assay assessed the expression levels of TNFR2, p65, p-p65, IKB-α, and p-IKB-α proteins in the NF-κB signaling pathway in cell lines transfected with NF-κB activator 1 and /or siADAMDEC1. C Representative images of BALB/c nude mice and tumor masses were taken on day 38 after inoculation with ADAMDEC1 cells, with or without shRNA-mediated silencing of ADAMDEC1. D The weight and volume curves of subcutaneous tumors in nude mice (*P < 0.05). E HE staining showed changes in cancer cells and intracellular structures. Ki67 expression in the siADAMDEC1 group was detected by IHC staining (**P < 0.01).

Article Snippet: The primary antibodies used in this study included GAPDH (Proteintech, 60004-1-Ig, 36 kDa), ADAMDEC1 (17899-1-AP, 44 kDa), TNFR2 (19272-1-AP, 70-75 kDa), p65 (10745-1-AP, 65 kDa), p-p65 (ab76302, 65 kDa), inhibitor of kappa B alpha (IκBα) (ab32518, 35 kDa), phosphorylated (p)-IκBα (ab133462, 35 kDa), MMP12 (ab52897, 54 kDa), and NF-κB activator 1 (HY134476, MCE).

Techniques: Expressing, Binding Assay, Western Blot, Transfection, shRNA, Staining, Immunohistochemistry

Clinical data in patients having diabetes mellitus (GI newly diagnosed DM, GII DM with metformin therapy) and control group

Journal: Journal of Diabetes and Metabolic Disorders

Article Title: A case-control study to determination FBXW7 and Fetuin-A levels in patients with type 2 diabetes in Iraq

doi: 10.1007/s40200-021-00738-x

Figure Lengend Snippet: Clinical data in patients having diabetes mellitus (GI newly diagnosed DM, GII DM with metformin therapy) and control group

Article Snippet: While HbA1c was determined using Bio- Rad VARIANT Hemoglobin A1c programmer.

Techniques: Control, Significance Assay

Correlation analysis of variables associated with serum FBXW7 protein level in the study populations

Journal: Journal of Diabetes and Metabolic Disorders

Article Title: A case-control study to determination FBXW7 and Fetuin-A levels in patients with type 2 diabetes in Iraq

doi: 10.1007/s40200-021-00738-x

Figure Lengend Snippet: Correlation analysis of variables associated with serum FBXW7 protein level in the study populations

Article Snippet: While HbA1c was determined using Bio- Rad VARIANT Hemoglobin A1c programmer.

Techniques: Control